(1) Healthy extracellular pH values are generally between 5.5 and 7.4. Both healthy and cancerous cells tightly regulate pH within the cell (intracellular pH) at typical values between 7.0 and 7.4. Thus, the synthesized novel nanomaterial shows a great potential for rapid visual imaging of cancer cells.Įxtracellular and intracellular sensing of pH are important tasks because of fluctuation of pH values with conditions such as cancer and bacterial infections.
Fluorescence imaging of cancer cells showed higher uptake of these nanoGUMBOS and more pervasive fluorescence intensity in comparison with normal cells. In this regard, in vitro fluorescence microscopy was employed to image cancerous and normal breast cells incubated with nanoGUMBOS. Peak ratios were significantly different at pH 5 and pH 7, indicating potential applications of this nanomaterial as a cancer diagnostic tool. The ratio of fluorescence emission corresponding to FL and RhB components of these nanoGUMBOS also changed with pH, allowing ratiometric analysis of pH through fluorescence measurements.
Nanoparticles prepared from this three-component GUMBOS, termed nanoGUMBOS, exhibited increased fluorescence at two wavelengths with increases in pH values. This sensor is composed of three ionic species, namely, fluorescein, rhodamine B, and tetradecyltrihexyl phosphonium (P 66614) ions.
Herein, we report the synthesis and characterization of a nanosensor developed from a group of uniform materials based on organic salts (GUMBOS).
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